A Pilot Study of the Primary Culture of the Oral Mucosa Keratinocytes by the Direct Explant Technique

نویسندگان

  • Gurkan Rasit Bayar
  • Yavuz Sinan Aydintug
  • Aydin Gulses
  • Pinar Elci
  • Meral Sarper
چکیده

Introduction: A reliable source of cultured keratinocytes is essential as a component of oral mucosa substitutes to treat burns and wounds of the oral and maxillofacial region. Primary monolayer cell cultures have been also extremely helpful in the study of the basic biology and responses to stimuli, of both oral and skin keratinocytes, and many studies have used them. There are two techniques in primary culture, the enzymatic and direct explant techniques. The direct explant technique has been used for 30 years in the culture of human gingival and buccal tissues and has appeared to be a successful technique in culturing human oral keratinocytes. In addition, it has been suggested that the direct explant technique obtains the first keratinocytes yield faster than the enzymatic technique. Aim: The aim of this pilot study is to present our experience in ex vivo production of oral mucosa keratinocytes by using the explant technique. Methods: The explant technique was used to cultivate oral mucosa keratinocytes. Results: The total success rate of primary culture of the oral epithelial cells by direct explant technique was 88.9%. No contamination of microorganisms in primary cell cultures was obtained. Conclusion: Within the limited numbers of samples used in the current pilot study, it can be concluded that the explant technique piloted in this study appears to be a simple and successful technique in the primary culture of oral mucosa keratinocytes. A larger study is required to confirm this finding.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ex vivo produced oral mucosa equivalent by using the direct explant cell culture technique.

OBJECTIVE The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique. MATERIAL AND METHODS Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were di...

متن کامل

Prevalence of diffused melanotic pigmentation of oral mucosa and related factors in dental students of Islamic Azad University of Medical Sciences, Tehran, 2018-2019

Abstract Background & Aim: The diffused melanotic pigmentation of oral mucosa is referred to any kind of brownish to black diffused and flattened macular and patch in physiologic or pathologic form. This view is important in aesthetic terms, the perception of malignancy, and association with systemic diseases, especially the endocrine glands diseases. Little local studies have been carried out ...

متن کامل

Human gingival fibroblasts culture in an autologous scaffold and assessing its effect on augmentation of attached gingiva in a pilot clinical trial

BACKGROUND AND AIM: An important goal of periodontal plastic surgery is the creation of attached gingiva around the teeth. In this study, the aims were to culture gingival fibroblasts in a biodegradable scaffold and measure the width of attached gingiva after the clinical procedure.METHODS: This study was carried out on 4 patients (8 sites), with inadequate attached gingiva next to at least two...

متن کامل

Interaction of primary mammary bovine epithelial cells with biofilm-forming staphylococci associated with subclinical bovine mastitis

Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...

متن کامل

Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering.

Reconstruction of large oral mucosa defects is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering using a fib...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011